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Using the Primer Database to store your lab’s collection of primers
Read more: Using the Primer Database to store your lab’s collection of primersMacVector’s Primer Database allows you to save and retrieve primers from a Primer Database with the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites using Primer Database Search. The tool comes with a starter database of primers, but you can use existing subsequence files or import primers from…
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Viewing external database entries for features in a sequence.
Read more: Viewing external database entries for features in a sequence.Sequences, or regions of sequences, can be linked to external databases. For example an entire sequence entry or for when annotation tools are used to annotate proteins with domain or motif information (e.g. InterProScan available in MacVector 15). Very useful for when you want to view more detailed or updated information. Within the Genbank specification,…
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Happy Holidays from all of us at MacVector…!
Read more: Happy Holidays from all of us at MacVector…!We wish you a happy, healthy and rewarding New Year!
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Optimizing the Reverse Translation function
Read more: Optimizing the Reverse Translation functionThe Analyze | Reverse Translation menu option lets you create a DNA sequence from a Protein sequence, reverse translated using a specific Genetic Code (by default, the Universal Genetic Code). The default option creates a DNA sequence with N’s and other ambiguities reflecting the degeneracy of the genetic code. This is great if you want…
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Tear-off Result Window Tabs: make viewing results easier.
Read more: Tear-off Result Window Tabs: make viewing results easier.All analysis results for an individual sequence are collected into a single tabbed result window to reduce window clutter. However, there are times when it is very convenient to have results displayed in side-by-side windows. For example, if you run a dot plot you can zoom in to view sections of the comparison by drag-selecting…
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IMPORTANT SERVICE ANNOUNCEMENT Entrez and BLAST services will stop working in November for MacVector 15.0.3 and earlier.
Read more: IMPORTANT SERVICE ANNOUNCEMENT Entrez and BLAST services will stop working in November for MacVector 15.0.3 and earlier.UPDATE – November 8, 2016 – Although the official switch off date is not until Wednesday the 9th, Entrez and BLAST are NOT currently working from MacVector 15.0.3 and earlier. We suspect this change is now permanent. UPDATE – November 7, 2016 – We have just been notified by the NCBI that they will be…
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Highlighting sequence using color and lower case in the Editor tab
Read more: Highlighting sequence using color and lower case in the Editor tabYou can very quickly annotate a region of interest in your sequence in the Editor tab. For example, showing introns in lower case or highlighting CDS features with a colored background. Using the TRANSFORMATIONS menu To enter sequences as mixed case. Enable Edit | Transformations | Enable Mixed Case Entry Type your sequence using SHIFT…
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Estimating insert length quickly for a read pair
Read more: Estimating insert length quickly for a read pair[Edit December 20, 2017 – As of MacVector 15.5 you can simply right click a READ and select “SEE MATCHING READS” to view the pair of reads. The total sequence length is selected. ] Insert length is the length of the sequence in between a pair of reads. Sequencers are supplied DNA samples in fragments of…
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Restriction enzyme analysis in MacVector and REBASE
Read more: Restriction enzyme analysis in MacVector and REBASEAlthough there are two different ways to perform restriction enzyme analysis with MacVector, there are also additional places where restriction enzyme sites are shown. All these tools use the same set of restriction enzyme files to recognise enzymes. These files are updated regularly from the REBASE database. The restriction enzymes are divided into multiple files.…
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Drag and drop to quickly annotate ORFs
Read more: Drag and drop to quickly annotate ORFsYou can use the Analyze | Open Reading Frames function to very quickly find ORFs on a sequence. Did you know that you can very quickly turn those results into permanent CDS features on your sequence? After running the Open Reading Frames analysis, simply drag and drop the ORF objects you are interested in from…
