Author: Chris
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MacVectorTip: Identifying, Selecting and Assembling NGS reads with a variant genotype
When analyzing/assembling/aligning NGS data, there are many scenarios where you might want to separate out the reads representing different genotypes or variant sequences. MacVector makes this very easy. Take a reference sequence and choose Analyze->Align to Reference. Now click the Add Seqs button and select and add your NGS data files. NOTE: if your reference…
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MacVectorTip: Filtering NGS Data to retrieve reads matching a known sequence
So you just got your NGS reads back from that sequencing experiment and, wow, what a HUGE amount of data. Wouldn’t it be easier to handle if you could pare that down to just the gene/plasmid/sequence(s) you are interested in? MacVector to the rescue as it can read and filter fast/q files, even if they…
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MacVectorTip: Use Bowtie to remove contaminating reads prior to NGS Assembly
MacVector with Assembler can use Velvet and/or SPAdes for fast and memory efficient de novo NGS assembly of modest sized genomes (typically up to 40 Mbp or so) even on a laptop. One common task is to assemble NGS data from BAC clones.However, one problem that often arises is that the BAC DNA preparations may…
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Applescript: batch translation of CDS features
Apple’s AppleScript (along with Javascript for Automation) is an easy to write and easy to understand language that allows you to easily automate tasks in supported applications. Many Apple applications have a AppleScript Dictionary that defines what functions you can automate. MacVector has many such functions in its AppleScript Dictionary. You can auto annotate multiple…
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MacVector 18.1 and the new InterProScan functional domain analysis tool
MacVector allows you to do functional domain analysis on your protein sequence using the InterProScan service. InterPro contains multiple databases of protein families, domains and motifs and InterProScan will submit a protein sequence to a search of these databases. It will also do extra analysis such as transmembrane region analysis using TMHMM and other tools.MacVector…
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MacVectorTip: Context-sensitive Menus in MacVector
Although Apple are well known (notorious?) for always providing mice with only a single obvious button, in reality the Mac interface from early versions of MacOS all the way to macOS Big Sur, plus many Mac apps, have always used right click menus (or more accurately “context sensitive menus”) to provide extra functionality. MacVector is…
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Make the most of your Apple Silicon Mac with MacVector 18.1
We are very pleased to announce that MacVector 18.1 is now available to download. MacVector 18.1 is a Universal Binary application, which means it runs natively on both Apple Silicon M1 Macs and Intel Macs. MacVector 18.1 matches the “Big Sur” look and feel. …and for the first time in many, many years the MacVector…
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MacVectorTip: Viewing genotype changes in Align to Reference assemblies
The latest releases of MacVector, MacVector 18.0.1 (Intel) and MacVector 18.1.1 (Intel and Apple Silicon) have some tweaks to the output of the SNPs tab in the Align to Reference assembly window. The genotypes of any SNP changes now follow a consistent standard, and short deletions are also reported. If the region containing the nucleotide…
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MacVector on Apple Silicon: benchmarks
MacVector 18.1 has just been released. MacVector 18.1 is a Universal Binary application, which means it runs natively on both Apple Silicon M1 Macs and Intel Macs. MacVector 18.1 also has graphical changes for a more authentic Big Sur look and feel. …and for the first time in many, many years the MacVector icon has…
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CRISPR tools in MacVector 18
If you are using CRISPR editing techniques then there are two useful functions in MacVector for you: Finding CRISPR Cas9 PAM sites. You can also use Scan DNA FOR.. tool to automatically show sites on all sequences. Screening sequences for CRISPR introduced INDELS