Category: Techniques
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Designing and testing primers in MacVector – part 2
In the first article in this series we took an overview of all the primer design tools in MacVector. In this second look at primer design in MacVector I’m going to look in more detail at our latest tool – Design Primers (Primer3). Primer3 is a powerful, highly configurable command line tool for designing primers.…
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An overview of Sequence Assembly in MacVector
MacVector has had sequence assembly functionality for a long time. Many moons ago MacVector came with a OS9 only tool called AssemblyLIGN. Although a useful tool it was fairly limited and needed a complete replacement, so about five years ago Assembler was released, which is a much more modern tool for sequence assembly. In addition…
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How to save a graphical Map.
With earlier versions of MacVector the graphical Map was treated as a separate object to the actual sequence, and you could save it directly as a graphic file (PICT) from the Map window. However, the graphical map changed substantially with MacVector 9.5 and even more in 10.0, and now you need a different way to…
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Designing and testing primers in MacVector – part 1
MacVector has many different primer tools for different purposes. In this, the first of a series of articles I want to present an overview of primer design in MacVector, and when it’s best to use which tool. I’ll also talk about what’s coming next in future releases. Design Primers (Primer3) The most flexible tool was…