-
MacVector 18.5 is macOS Ventura ready
Read more: MacVector 18.5 is macOS Ventura readyIt’s that time of year again. Apple have just released macOS Ventura and we are very pleased to announce our current release, MacVector 18.2 has been tested on macOS Ventura and is compatible. Not only that but we have just a few weeks of testing left before our next release. MacVector 18.5 has been developed…
-
MacVector is macOS Ventura ready
Read more: MacVector is macOS Ventura readyIt’s that time of year again. Apple have just released macOS Ventura and we are very pleased to announce our current release, MacVector 18.2 is macOS Ventura ready. Not only that but we have just a few weeks of testing left before our next release. MacVector 18.5 has been developed on macOS Ventura since the…
-
MacVectorTip: Create custom Codon Usage Tables for ORF analysis and reverse translation
Read more: MacVectorTip: Create custom Codon Usage Tables for ORF analysis and reverse translationMacVector uses codon usage tables (also called “Codon Bias”) to help you predict protein coding regions in the Analyze | Nucleic Acid Analysis Toolbox functions and also the protein Analyze | Reverse Translation… function. For example, here are the Plus and Minus strand Staden Codon Preference plots aligned under the Plus/Minus ORFs for a Streptomyces…
-
macOS’s tabbed windows and MacVector
Read more: macOS’s tabbed windows and MacVectorOne of the lesser known features of macOS is the ability to store all open documents of an application in tabs. Tabs were initially introduced for the Finder, but macOS Mavericks saw them apply to supported application document windows too. MacVector has supported tabs since their introduction, however, by default the Tab Bar is turned…
-
MacVectorTip: Identifying, Selecting and Assembling NGS reads with a variant genotype
Read more: MacVectorTip: Identifying, Selecting and Assembling NGS reads with a variant genotypeWhen analyzing/assembling/aligning NGS data, there are many scenarios where you might want to separate out the reads representing different genotypes or variant sequences. MacVector makes this very easy. Take a reference sequence and choose Analyze | Align to Reference. Now click the Add Seqs button and select and add your NGS data files. NOTE: if…
-
MacVectorTip: Trimming trace files by quality
Read more: MacVectorTip: Trimming trace files by qualityMany of our users are familiar with the ability of Sequencher to semi-automatically trim poor quality sequences from the ends of Sanger ABI reads. Although it is generally not necessary to do this in MacVector because most of the algorithms can automatically handle poor quality data, there are times when it can be beneficial. So…
-
MacVectorTip: How to copy a specific short amino acid translation of a sequence
Read more: MacVectorTip: How to copy a specific short amino acid translation of a sequenceThere can be times when you are messing around with open reading frames, inserting residues to change frames to try to get the perfect CDS fusion. The MacVector single sequence Editor will show those (click and hold on the “Display” toolbar button) but if you select and copy, only the DNA sequence (with any overlapping…
-
Simulating DNA electrophoresis in agarose gels using MacVector’s Agarose Gel tool
Read more: Simulating DNA electrophoresis in agarose gels using MacVector’s Agarose Gel toolMacVector has a Agarose Gel interface which allows you to view photo-realistic recreations of restriction digests of linear and circular DNA molecules. The gels look so realistic that users have had a hard time telling photos of their own digests from the simulation in MacVector. When you first use the new tool and compare it…
-
MacVectorTip: How to find Restriction Enzymes that only cut outside of a specific region
Read more: MacVectorTip: How to find Restriction Enzymes that only cut outside of a specific regionOne common cloning related task is to ask MacVector to find restriction enzyme sites that cut in a molecule, but that do not cut in a specific region. e.g. suppose you want to find restriction enzymes that cut pBR322 but that do not cut in the Tetracycline Resistance Gene. To do this, choose the Analyze…
-
MacVectorTip: Simulating mixed plasmid populations in agarose gels
Read more: MacVectorTip: Simulating mixed plasmid populations in agarose gelsWe had a recent support call this week from somebody who believed from their agarose gels that they had a mixed population of plasmids from an experiment and wanted to document and determine the banding pattern using MacVector’s agarose gel simulation. You might come across this type of scenario if you have been making site-specific…
